Journal: Sensors (Basel, Switzerland)

Article Title: Denoising Algorithm for High-Resolution and Large-Range Phase-Sensitive SPR Imaging Based on PFA

doi: 10.3390/s25154641

Figure Lengend Snippet: Protein antigen–antibody interaction curves between goat anti-rabbit IgG and rabbit IgG. ( a ) Denoised SPR curves and the fitted curves of the whole ROI in the proposed SPR system and denoised frame. ( b ) Associating and dissociating curves measured by Biacore 8 K. ( c ) Denoised SPR curves and the fitted curves of each ROI in the proposed SPR system and denoised frame. ( d ) ROIs are shown in the phase image and prism. ( e ) Denoised SPR phase signals of a single ROI across multiple tests in deionized water. The solid line represents the typical curve, while the light-colored lines show results from repeated experiments.

Article Snippet: The system’s performance is validated through ultra-dilute NaCl solution switching (0.0025–0.08%) and biomolecular interaction studies, accurately quantifying antibody–protein binding kinetics down to 0.15625 μg/mL, with an equilibrium dissociation constant (KD) of 1.97 × 10 −9 M, consistent with Biacore 8 K, the commercial SPR scientific instrument.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: A Bifunctional SARS-CoV-2 Entry Inhibitor Targeting the Host Protease TMPRSS2 and Viral Spike Protein HR1 Region

doi: 10.3390/ijms26178289

Figure Lengend Snippet: Interaction of IP4X and IP4Z with TMPRSS2. ( A ) TMPRSS2 inhibition by chimeric inhibitors and peptide IPB19. Experiments were conducted with 5 nM recombinant TMPRSS2 and 100 µM Boc-QAR-AMC peptide substrate, and the data are expressed as means ± the standard deviation (error bar) for assays performed in two technical replicates. IC 50 values were calculated with GraphPad Prism 8 software. SPR analysis of ( B ) Camostat, ( C ) IP4X, and ( D ) IP4Z with TMPRSS2. K D values were determined using Biacore 8K evaluation software version 1.

Article Snippet: SPR analysis was performed on a Biacore 8K instrument (GE Healthcare, IL, USA).

Techniques: Inhibition, Recombinant, Standard Deviation, Software